MOLECULAR IDENTIFICATION AND evolution OF monocot genus SPECIES FROM NORTH-EASTERN Bharat BY INTERNAL TRANSCRIBED SPACER two OF NUCLEAR deoxyribonucleic acid

Abstract

The internal transcribed spacer region of the ribosomal RNA sequence cluster within the nuclear polymer has been used mutually of the foremost informative molecular markers for identification and organic process analysis of fungi and plants in the last 3 decades. The aim of this study was to guage the appliance of ITS2 locus of rRNA region in nuclear polymer as molecular marker within the identification and molecular evolution of fifteen genus Musa specimens (Musaceae) in North-Eastern Asian nation. The sequence characteristic of the ITS2 locus disclosed a length variation from a minimum of 210 bp in genus Musa mannii to a most of 223 bp in M. balbisianawith a median of 214 bp. The rate contents of the ITS2 locus conjointly varied from a minimum of sixty four.95% in M. markkui to seventy.97% in M. balbisiana with a median of sixty nine.01%. Sequence similarity analysis of the ITS2 regions disclosed sequence similarity from a minimum of ninetieth for M. markkui followed by ninety seven for M. acuminata and ninety eight for M. nagensium whereas the remaining species showed ninety nine to a thousandth similar sequence identities publically info. The organic process analysis supported ITS2 sequence disclosed a monophyletic origin of all taxa underneath totally different genera of the family Musaceae. 

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Posted March 8, 2019 07:06