Latest Biotechnology Research

Research No-1

EVALUATION OF RS2165241 SNP IN EXFOLIATION GLAUCOMA PATIENTS OF KHORASAN PROVINCE

 One of the most important causes of exfoliation glaucoma is the exfoliation syndrome in which lysyl oxidase-like 1 (LOXL1) gene most-strongly associated with exfoliation glaucoma. The rs2165241 was located in intron 1 of LOXL1 gene and it is believed that rs2165241 could alter LOXL1splicing. The objective of this comprehensive study was the association of rs2165241 single nucleotide polymorphisms (SNP) in patients with exfoliation glaucoma and control individuals. Sixty unrelated exfoliation glaucoma (XFG) on the Iranian’s patients, as well as 40 control subjects were studied for a LOXL1 gene polymorphism (rs2165241) by using PCR sequencing. According to the results of this study, there is significant associations between TT genotype of rs2165241 SNP and XFG (P<0.0001); the T allele for rs2165241 was more frequent in patients compared to control subjects (P<0.0001), and C allele was a factor of the utmost importance in modifying XFG. Thereby, patients with TT as well as TC genotypes had a high potential for XFG disease. The clinical data in case and control group showed that IOP in left and right (p= 0.019 and p=0.032, respectively) was significantly correlated with XFG. Consequently, the findings of this research declared the good agreement between the relationship of rs2165241 SNP and XFG disease in Iranian’s patients.

Research No-2

PHYTOSOCIOLOGICAL STUDIES ON URANIUM DEPOSITS OF BHILWARA DISTRICT

 Uranium deposits have been found to occur in Dhor and Tikhi Ka Jhonpara in Jahazpur area of Bhilwara district. Atomic Mineral Directorate for Exploration and Research Department (Western region) is working there for mining of uranium. Phytosociological studies were carried out in those areas. It is interesting to note that Rhus mysoresis and Anogessius pendula have highest fidelity, constancy, sociability and cover-abundance values in Dhor and Tikhi Ka Jhonpara repectively. So these plant species might be "Characteristic species" in those areas.

Research No-3

GAMMA RADIOSENSITIVITY STUDIES ON ZINNIA ELEGANS VAR DREAMLAND

 The success of induced mutagenesis technique using gamma rays in any crop or ornamental plants lies in the determination of optimum dose (LD₅₀). The study aims to determine the optimum dose of gamma rays in Zinnia elegans var Dreamland and the effect of different doses of gamma radiation on the germination and survival rate, plant height and root length. The seeds were treated with 10Gy, 20Gy, 50Gy, 75Gy, 100Gy, 125Gy, 300Gy, 400Gy and 500Gy at Bhabha Atomic Research Centre, Mumbai using ⁶⁰CO source. The results revealed that there was significant decline in the germination rate after ⁵⁰Gy gamma radiation dose and no significant variation in the survival rate until 300Gy as compared to the control. The LD50 for % germination and %survival was found to be 155.98 and 250.78. The higher gamma ray doses significantly decreased the plant height and root length. 40% reduction in shoot length and root length was found to be 250 to 260 and 210 to 220.

Posted August 20, 2018 08:43