UntitledDIAGNOSIS OF CYSTIC HYDATIDOSIS IN EXPERIMENTALLY INFECTED SHEEP BY ELISA

Abstracts

 Aim: Cystic echinococcosis, a zoonotic infection, is caused by metacestod formation of the Echinococcus granulosus, and it causes major economical loses and health disorders. This study was carried out to determine the sensitivities of ELISA test in order to serologic diagnosis of cystic echinococcosis in experimentally infected sheep.

Materials and Methods: A total of 20 Akkaraman sheep (4-5 months) were subjected in this study as 10 for control and another 10 for experimental group. A total of 15.000 live protoscoleces collected from liver with hydatid cyst were administered into a dog via oral route, and at the end of two months, maturated E. granulosus was collected from intestinal lumen. Approximately 2000 eggs were administered into each sheep in the experiment group. Sheep had been observed for 12 months. During this period, all the sheep were checked by using ELISA for parasite specific antibodies.

Results: ELISA test used with partially purified cyst fluid antigen had shown 57% sensitivity and 67% specifity when compared to necropsy. Antibody responses in sheep during 12 months were shown different peaks in different times. The foremost antibody response was determined in two weeks after inoculation. No antibody response was determined in calcified cysts.

Conclusion: It may be stated that ELISA test are more reliable for the diagnosis of cystic echinococcosis, and necropsy findings can be used as reference for determination of sensitivity and specifity of serology.

Keywords :

ELISA, cystic hydatidosis, sheep.

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Posted July 5, 2018 05:08